Ordering information

• Oligonucleotide Request
• siRNA Request

Oligo orders can still be dropped off at the facility. Turnaround time for 40 nmole orders averages 2 days. We will email you when your oligo is ready.

Please use a separate order form for each oligonucleotide. If you need a form follow the link below and print a copy.

Click here to dowload the Request Form in MS-Word format

Please fill out the form carefully according to the following instructions:

Name: Requestor's name.
Building/Room#: Location of the requestor's laboratory. If this is your first order, please provide us with your department name and campus box address.
Billing PI (Full Name): Name of the principle investigator to whom the bill should be sent.
Billing Account Number: 6-digit number of the billing account, i.e.: 6-54321
Email address and phone # : We will email you or call this number when the oligo is ready.
Date: Date placing the order.
Length: ___mer Number of bases in the requested oligo.
Sequence (In Triplets 5' -->3') Please write clearly using A, g, C, T or the IUB degeneracy codes shown at the bottom of the order form. There is no charge for degeneracies in positions other than the 3' end.
List Modifications: If you have included any non-ATgC nucleotides in your sequence, or if you are requesting end labeling with biotin, fluorescein, phosphate, etc, indicate them here and in your sequence. Click here to see a list of modifications and their prices.
Optional Purification: If you want us to carry out "Trityl On" purification ($15 additional charge per oligo) or SepPak desalting (free), circle as appropriate. "Trityl On" is unavailable for 40 nM scale oligonucleotides more than 50 base pairs.
Designation: Your name for the oligo. Please make this 8 characters or less.
Scale of Synthesis: Scale refers to the theoretical molar yield of the synthesis, although in most cases the actual yield of a synthesis is significantly lower. The approximate molar yields for each of the available synthesis scales are as follows:

Synthesis

The 40 nmole crude oligos are suitable for most applications including sequencing, PCR and cloning. A 25-mer synthesized at this scale will provide about 250 µg of crude DNA.

Final products:
For desalted and purified oligos: yield of the oligo is measured in OD unit, µg and nmol. (check the bottom of your request form).
For crude oligos -- check the concentration by spectrophotometer.