Oligo orders can still be dropped off at the facility.
Turnaround time for 40 nmole orders averages 2 days. We will email
you when your oligo is ready.
Please use a separate order form for each oligonucleotide. If you need
a form follow the link below and print a copy.
Click here to dowload the Request Form in MS-Word format
Please fill out the form carefully according to the following instructions:
Name: Requestor's name.
Building/Room#: Location of the requestor's laboratory. If this
is your first order, please provide us with your department name and campus
Billing PI (Full Name): Name of the principle investigator to
whom the bill should be sent.
Billing Account Number: 6-digit number of the billing account, i.e.: 6-54321
Email address and phone # : We will email you or call this number
when the oligo is ready.
Date: Date placing the order.
Length: ___mer Number of bases in the requested oligo.
Sequence (In Triplets 5' -->3') Please write clearly using
A, g, C, T or the IUB degeneracy codes shown at the bottom of the order
form. There is no charge for degeneracies in positions other than the
List Modifications: If you have included any non-ATgC nucleotides in your
sequence, or if you are requesting end labeling with biotin, fluorescein,
phosphate, etc, indicate them here and in your sequence. Click
here to see a list of modifications and their prices.
Optional Purification: If you want us to carry out "Trityl
On" purification ($15 additional charge per oligo) or SepPak desalting
(free), circle as appropriate. "Trityl
unavailable for 40 nM scale oligonucleotides more than 50 base pairs.
Designation: Your name for the oligo. Please make this 8 characters
Scale of Synthesis: Scale refers to the theoretical molar yield of the
synthesis, although in most cases the actual yield of a synthesis is significantly
lower. The approximate molar yields for each of the available synthesis
scales are as follows:
The 40 nmole crude oligos are suitable for most
applications including sequencing, PCR and cloning. A 25-mer synthesized
at this scale will provide
about 250 µg of crude DNA.
For desalted and purified oligos: yield of the oligo is measured in OD unit,
µg and nmol. (check the bottom of your request form).
For crude oligos -- check the concentration by spectrophotometer.