Sample Submission

 

Please note: Samples accepted by APPOINTMENT ONLY. We are sorry for this inconvenience but we have found it extremely useful to discuss your submission in person. A discussion with the Proteomics Core staff will increase the likelihood that your samples will be properly analyzed and useful data will be obtained. This may only take a few minutes depending on the complexity and clarity of the task. An appointment is necessary because we are not always available especially when we are involved with our other tasks.

 

Radioactive samples ARE NOT ACCEPTED

 

Sample submission is a three step process

1)  Fill out the sample information form (link) and save to your computer.

2) Go to our online submission page.

Go to this online submission page if you are a UNC employee.

Go to this online sumission page a if you are a UNC Affiliate, Academic or Corporate customer.

i) clink on link.

ii) Specify the type of customer.  The UNC option is only for UNC-Chapel Hill customers.  Individuals from other UNC campuses, pick academic.

iii) Fill out services requested.

iv) Attach sample information form, any sequences, or images.

v)  Submit order.  On the final page of submission, you will be required to provide either an account number (internal customers) or PO number (external customers).

3) Schedule appointment with us to bring samples.

 

For protein identification (PID) from a gel band or spot:

Contact Info: Ms. Nely Dicheva Glaxo Building,

Room 111, Glaxo Building

Phone: (919) 843-2589 or (919) 966-9990

Email

 

For Analysis of a complex Mixture by LC-MALDI-TEMPO

Contact Info: Dr. David Smalley

Room 111, Glaxo Building

Phone: (919) 966-8318

Email

 

For analysis of post-translation modifications by LC-ESI-LTQ-Orbitrap, for all other submission, or if you are unsure.

Contact Info: Dr. David Smalley

Rm 111, Glaxo Building

Phone: (919) 966-8318

Email

 

For all projects and more complex questions, feel free to contact:

David Smalley Phone: (919) 966-8318 Email

OR

Dr. Lee Graves Phone: (919) 966-0915 Email

 

Note: If you will be using FedEx to send samples to the core, please view these shipping instructions.

 

If you are not an experienced user of our services, we recomment that you read the Methods and Advice Page.

 

***General Advice about submission of a gel. We recommend gels be stained with either Commassie Blue or Sypro Ruby. These produce the best results. Stains with colloidal coomassie G-250 generally produce “better stains”, most common commassie blue stains are capatible with mass spectrometry. Silver stained gels generally produce less robust results because they modify the proteins and crosslink the peptides to the gels. If you need to use silver stains, they must be mass spec compatible. Most commercial suppliers will indicate this on their product insert. We recommend this one (link).

 

All protein identification services include searches of publicly available databases and a results report. Database searches of client-provided sequences or specific modifications are available for an additional charge.