Next Generation Sequencing and Genotyping

Leadership

William Marzluff, PhD, Faculty Advisor

Corbin Jones, PhD (NextGen); William Marzluff, PhD (DNASeq), Michael Topal, PhD (Genotype), Faculty Directors

Piotr Mieczkowski, PhD (NextGen); Laura Livingstone, PhD (DNASeq), Jingchun Luo, MD,MS (Genotype), Facility Directors

Services

Next Generation Sequencing

Provides comprehensive Next-Generation high-throughput sequencing services via eight (8) Illumina Genome Analyzers and a Roche/454 Titanium. Four of these have been upgraded to the HiSeq 2000. Four HiSeq 2000 will be added in August 2010 and a Pacific Biosciences SMRT DNA sequencing system in Fall 2010.

Technologies offered:
• Whole Genome Shotgun Sequencing
• ChIP-seq
• FAIRE-seq
• RNA-seq
• SmallRNA-seq
• Microbiome surveys
• Post-capture exon-surveys


DNA Sequencing
The DNA Sequencing component continues to operate two 3730xl 96-capillary DNA Analyzers. The following services are offered:

• Sequencing of DNA samples on the ABI 3730xl DNA Analyzer, including a long read (1000 bases) sequencing options

• Low cost high throughput DNA sequencing in sets of 95 samples (96 well plate format) using the ABI 3730xl DNA Analyzer.

• Provision of standard vector primers known to work well in DNA sequencing.

• Custom sequencing for reading through difficult regions of sequence using alternative sequencing chemistries and protocols.

Read more about DNA Sequencing.

High Throughput Genotyping
The component offers an array of SNP Genotyping services including:

• TaqMan standard and custom SNP genotyping: 1 SNP on each sample at a time.

• GoldenGate custom SNP genotyping: from 96 to 1,536 SNPs on each sample at a time.

• Select Infinium custom panels: From 3,000 to 200,000 SNPs.

• Whole Genome Genotyping from 300,000 to 1,200,000 SNPs.
Human copy number analysis: DNA copy number variations (CNVs) and loss of heterozygosity (LOH) analysis using the Illumina Infinium Genotyping BeadChips.

Read more about High Throughput Genotyping.

Contact Information

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