Holden Thorp, Ph.D.

Professor
Non-aligned

Research Interests
Our research centers on understanding chemical reactions of nucleic acids with small molecules, especially transition metal complexes. Such reactions provide significant insight into the mechanisms of DNA damage, mutagenesis, and the action of many chemotherapy drugs. In addition, we have used these reactions to develop new methods for detecting nucleic acids in clinical samples based on hybridization and electrochemistry. These methods are being applied to the analysis of gene expression in tumors by monitoring the quantity of mRNA for a specific gene. Recently, we have applied these methods and principles to understanding the structure and function of telomeric DNA and find that oxidation of guanine in telomeres affects telomerase function and the activity of telomerase inhibitors. A significant new direction for our laboratory is the development of ionic liquids consisting of nucleic acids and redox-active metal complexes that are functionalized to induce formation of liquid phases. These new "liquid DNA" materials offer new methods for miniaturization of biomolecule devices and may be of use in highly multiplexed arrays.


Publications
Detection of Attomole Quantities of DNA Targets on Gold Microelectrodes by Electrocatalytic Nucleobase Oxidation. M. S. Gore, V. A. Szalai, P. A. Ropp, I. V. Yang, J. S. Silverman, H. H. Thorp* Anal. Chem. 2003, 75, 6586-6592.

Metal Ligand Charge-Transfer Promoted Photo-Electronic Bergman Cyclization of Copper Metallo-Enediynes: Photochemical DNA Cleavage Via C-4' H-Abstraction. P. J. Benites, R. C. Holmberg, D. S. Rawat, B. J. Kraft, L. J. Klein, D. G. Peters, H. H. Thorp,* J. M. Zaleski* J. Am. Chem. Soc., 2003, 125, 6434-6446.

Site-Specific Probing of Oxidative Reactivity and Telomerase Function Using 7,8-Dihydro-8-Oxoguanine in Telomeric DNA. V. A. Szalai, M. J. Singer, H. H. Thorp* J. Am. Chem. Soc. 2002 124, 1625-1631.

An Ionic Liquid Form of DNA: Redox-Active Molten Salts of Nucleic Acids. A. M. Leone, S. C. Weatherly, M. E. Williams, R. W. Murray*, H. H. Thorp* J. Am. Chem. Soc. 2001, 123, 218-222.


Click here for a list of Publications on PubMed

E-mail: holden@unc.edu
Telephone: (919) 962-0276
FAX: (919) 962-2476
Address: A402 Kenan Labs, CB# 3290 Chapel Hill, NC
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